The direct and short-term effects of recombinant human growth hormone on osteoblasts in vitro were observed.
METHODS: Different concentrations of recombinant human growth hormone (RHGH) were added to rat osteoblast culture system to observe the proliferation, alkaline phosphatase activity and cell cycle of rat osteoblasts at different time points. influences.
RESULTS: RHGH had an effect on osteoblast proliferation and alkaline phosphatase activity. It promoted osteoblast proliferation, increased alkaline phosphatase activity, and increased the percentage of cells in S phase and G2/M phase in the cell cycle. The intensity of its action increases with increasing concentration.
Conclusion: RHGH has a direct effect on the anabolism of osteoblasts, and has a stronger effect on osteoblast proliferation and alkaline phosphatase activity than estrogen.