The specific composition consists of the following cycles:
The columns and monomers protected by Fmoc must remove the amino protective group with an alkaline solvent (piperidine).
Activation and cross-linking
The base of the next amino acid is activated by an activator.
Chemical processes are commonly used by HBTU/HCTU/HITU/HATU-NMM/DIPEA or HOBT-DIC as activators, activating monomers in cross-linking with free ammonia reactions to form peptide bonds.
HPLC Analysis Purification The analysis of hPLC using a column and pump system can withstand high pressure, which can be filled with very fine particles (3-10 m).